FAM Annexin V PI Apoptosis Detection Kit


Cat No. MB521-0100 Size: 100 Reactions
Cat No. MB521-0025 Size: 25 Reactions
Store at 4°C in the dark for 1 year
Description
The GeneDireX FAM annexin V PI apoptosis detection kit is designed for detecting cell apoptosis. In normal health cells, phosphatidyl serine (PS) is located on the cytoplasmic surface of the cell membrane. However, in apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human anti-coagulant, annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. The annexin V is labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. The Propidium iodide (PI) is a common fluorescent dye to detect DNA. It can be used in flow cytometry to evaluate the cell cycle and cell viability during apoptosis. The FAM annexin V PI apoptosis detection kit can be used in one-step staining procedure without wash step within 20 minutes.
Feature

  • High speed- Within 20 minutes
  • Master mix format with fluorescent dye—minimizes possibility of cross- contamination, reduces sample handling
Applications

  • Clinical diagnosis
  • Knockout analysis
Kit Content
Catalog number MB521-0100 MB521-0025
FAM Annexin V 1 X 0.5 mL 1 X 0.125 mL
Propidium Iodide (PI) 1 X 0.5 mL 1 X 0.125 mL
10X Binding Buffer 3 X 2 mL 1 X 2 mL
Quality Control
The quality of the FAM annexin V PI apoptosis detection kit is tested on a lot-to-lot basis to ensure consistent product quality.
Required Materials

  • Centrifuge tube
  • Cold phosphate buffered saline (PBS)
  • Flow cytometry
  • Flow cytometry tube
Protocol
MSDS