GScript First-Strand Synthesis Kit


Cat No. MB305-0050 Size: 50 Reactions
Description
The GScript First-Strand Synthesis Kit is a recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has a lower RNase H activity and a high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb. The GScript RTase has a special activity with its secondary structure, rendering stabilization during the reverse transcription reaction.
Kit Contents
Component Volume (μL)
Oligo(dT)20(50 μM) 50
5X 1st strand buffer 250
DTT (0.1 M) 100
dNTP mix (10 mM) 50
GScript RTase (200 U/ μl) 50
Quality Control
The quality of the GScript First-Strand Synthesis Kit is tested on a lot-to-lot basis to ensure consistent product quality.
Protocol
MSDS