ITS-M, 500X


Cat. No.: CC002-1000 Size: 1 ml
Storage: 4°C
Description
The GeneDirex ITS-M is a mixture of insulin, transferrin, and sodium selenite. It is a general cell supplement designed for use in classical media such as DMEM, RPMI-1640 and nutrient media such as Ham’s F-12 or DMEM/F12.
They stimulate cell proliferation of a variety of cells under serum-reduced conditions, enhance cell proliferation, and decrease the serum requirement of many cell types.
Insulin is a hormone that promotes glucose and amino acid uptake by the cell. It is thought that the mitogenic effects of insulin are due to the insulin-like growth factor receptor, IGF-1 receptor. Transferrin is an iron transport protein that functions to transport iron into the cell. The protein also serves to detoxify the medium from oxygen radicals and peroxidase. Selenium is an enzyme cofactor that activates glutathione peroxidase, a player in the detoxification of oxygen radicals.
When ITS-M is used with a low percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum. Supplementation with ITS-M allows for a reduction in the serum requirement of the culture.
Working Concentration:
1 ml vial of Insulin-Transferrin-Selenium Mixture is sufficient for 500 ml of medium. In general, it is necessary to add 1% serum to achieve optimal growth; some adherent cultures may require 2% serum supplementation.
Formulation
Component Concentration (g/L)
Insulin 5.00
Transferrin 2.75
Sodium Selenite 0.00335
Sodium Pyruvate
Ethanolamine
* Prepared in EBSS without Phenol Red.
Applications
Supplement of cell culture.
Caution
1. During operation, always wear a lab coat, disposable gloves, and protective equipment.
2. Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.
Protocol
MSDS