OnePCR Plus


Cat No. MB207-0100 Size: 100 Reactions ( 2 × 1.25ml )
Cat No. SM207-0100 Size: 100 Reactions ( 2 × 1.25ml )
Description
OnePCR Plus is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR Plus is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTP, gel loading dyes, enhancer, and fluorescence dye. OnePCRTM Plus which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR Plus, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epiilluminator after the DNA electrophoresis. OnePCR Plus mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.
    
Application

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No de-staining requirement.
  • Compatibility – Use the blue light or UV to detect the signal.
Storage
Store at RT up to 3 month. Store at 4°C up to 6 month. Store at -20°C up to 1 year. Shipping Temperature: 4°C
Note: OnePCR Plus is light sensitive and should be stored and protected from light.
Download
MB207-0100 Protocol
MSDS
SM207-0100 Protocol
MSDS