OnePCR HotStar


Cat No. MB206-0100 Size: 100 Reactions ( 2 × 1.25 ml )
Description
OnePCR HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR HotStar is a pre-mixed solution containing Hot start Taq DNA polymerase, PCR Buffer, dNTPs, gel loading dyes, and fluorescence dye. OnePCR HotStar, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. Hot start Taq DNA polymerase has a non-template–dependent terminal transferase activity that adds a 3’ deoxyadenosine to product ends, and has a 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). OnePCR HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions.
Application

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No de-staining requirement.
  • Compatibility – Use the Blue Light or UV to detect the signal.
Storage
Store at RT up to 1 month.
Store at 4°C up to 6 month.
Store at -20°C up to 1 year.
Shipping Temperature: 4°C
Note: OnePCR HotStar is light sensitive and should be stored and protected from light.
Protocol
MSDS