OneTwin 100


Kit contents

Component Volume Cat No.
OnePCR 1.25ml MB203-0050
OneMark 100 600ul DM101-0100

OnePCR

Description
OnePCR is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.
OnePCR which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Reagents are provided with the sufficient amplification reactions of 50 μl each.

Application

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No de-staining requirement.
  • Compatibility – Use the blue light or UV to detect the signal.

Size: 50 Reactions
Storage
Store at RT up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping Temperature: 4°C
Note: OnePCR is light sensitive and should be stored and protected from light.

OneMARK 100

Description
OneMARK 100 with the Novel Green was designed to show virtually uniform spacing over a wide fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking dyes. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes, ethidium bromide or SYBR® Green I.
The OneMARK 100 with the fluorescent stain was optimized for direct loading onto unstained agarose gels.
The ladders provide highest level of convenience during the routine handling and avoid commonly used gel staining procedures with ethidium bromide or SYBR® Green I.
The OneMARK 100 includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 μg per loading) for approximating the mass of DNA in comparably intense samples of similar size.

Application

  • No-post-staining procession
  • Direct loading onto your agarose gel for analysis

Source
PCR products and double-stranded DNA digested with appropriate restriction enzymes are phenol-extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Note:
OneMARK 100 is light sensitive and should be stored and protected from light.

Range: 100-3,000 bp

Number of bands: 12

Concentration: 90 μg/ml

Package: 54 μg /600 μl

Recommended Load: 6 μl / well
Containing orange G, xylene cyanol FF as the tracking dyes.

Storage
Store at RT and 4°C up to 6 month.
Store at -20°C up to 1 year.

Size:600 μ

Protocol
MSDS