Plasmid midi PREP Kit


Cat No. NA205-0020 Size: 20 Reactions
Description
The Plasmid midiPREP Kit provides a fast, simple, and cost-effective Plasmid midiPREP method for isolating the plasmid DNA from the cultured bacterial cells. The Plasmid midiPREP Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid midiPREP Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.
Features

  • Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride,  thus minimizing the exposure to and disposal of hazardous materials.
  • Time saving: Complete the process in less than 40 minutes.

 

Protocol
MSDS