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OnePCR

$50.00 $45.00

Description
OnePCR is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.
OnePCR which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Reagents are provided with the sufficient amplification reactions of 50 μl each.

SKU: MB203-0100 Category:

Description

Cat No. MB203-0100 Size: 100 Reactions
Description
OnePCR is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.
OnePCR which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Reagents are provided with the sufficient amplification reactions of 50 μl each.
Application

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No de-staining requirement.
  • Compatibility – Use the blue light or UV to detect the signal.
Storage
Store at RT up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping Temperature: 4°C
Note: OnePCR is light sensitive and should be stored and protected from light.
Mammalian Chromosomal Aberration Test
Mutation Test Report
Acute Toxicity Report
Protocol
MSDS