Ultra293™ Transfection Reagent


Cat. No. MB506-1000 Package: 1 ml
Cat. No. MB506-0100 Package: 100 ul
Store at 4°C (Do not freeze)
Description
The GeneDireX Ultra293™ is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. It provides all the attributes of the trusted transfection reagents: high transfection efficiency, low toxicity, simplicity of use and reproducibility. Ultra293™ is suitable for both transient and stable transfection and can be used for many applications. After Ultra293™ and plasmid mixed, the Ultra293™/ plasmid complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells. It provides effectively, reproducibly, and affordable benefits for scientific research. The entire procedure can be completed in 25-30 minutes.
Note

  • Maintain the same seeding conditions between experiments.
  • For most cell lines, transfection in the absence of serum is optimal. For HeLa cells, high level transfection requires the presence of serum in the medium during transfection. This option should be tested if optimization does not yield high-level transfection.
  • Different cell types and number of passages might lead to different transfection efficiency, and we recommend using at least two different concentrations of transfection reagent as control in new transfect experiments to optimize experimental conditions.
  • Tranfect cells at 50-60% confluency is recommended.
  • To lower cytotoxicity, replace Ultra293/DNA complex-containing medium 4 to 12 hours post-transfection. (Optional)
Kit Contents

Component MB506-1000 MB506-0100
Ultra293™ 1000 (ul) 100 (ul)
Required Materials
Cell line
CO2 incubator
Reduce serum medium without serum
 Quality Control
The quality of the GeneDireX Ultra293 is tested on a lot-to-lot basis to ensure consistent product quality.
Protocol
MSDS