Ultrafect™ Reagent System


Cat. No. MB502-1000 Size : Ultrafect Reagent: 1 ml TransPlus Reagent: 1 ml
Cat. No. MB502-0100 Size : Ultrafect Reagent: 100 ul TransPlus Reagent: 100 ul
Store at 4°C (Do not freeze)
Description
Ultrafect carries most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. It delivers superior transfection efficiency and improved cell viability.
Note

  • Do not add the antibiotics to the medium during transfection as this causes cell death.
  • Maintain the same seeding conditions between experiments.
  • For most cell lines, trans-fection in the absence of serum is optimal. For HeLa cells, high level trans fection requires the presence of serum in the medium during transfection. This option should be tested if optimization does not yield high-level transfection.
  • Some no serum medium might inhibit the transfection of cation ion and plasmid. We recommend using the reduced serum medium without serum.
  • Different cell types and number of passages might lead to different transfection efficiency, and we recommend using at least two different concentrations of transfection reagent as control in new transfect experiments to optimize experimental conditions.
Kit Contents
Catalog number MB502-1000 MB502-0100
UltraFect Reagent 1 ml 100 ul
TransPlus Reagent 1 ml 100 ul
Required Materials

Cell line
CO2 incubator
Reduce serum medium without serum

Quality Control
The quality of the GeneDireX Ultrafect™ Reagent System is tested on a lot-to-lot basis to ensure consistent product quality.
Protocol
MSDS