amaR OnePCR HiFi

Cat No. SM215-0250 Size: 250 Reactions (2 X 1.25 ml)
Cat No. SM215-0010 Size: 10 Reactions (1 X 100 µl)
Storage
– Store at RT up to 1 months.
– Store at 4°C up to 6 months.
– Store at -20°C up to 1 year.
Note: amaR OnePCR HiFi is light sensitive and should be stored and protected from light.
Description

The amaR OnePCR HiFi is a ready-to-use PCR reaction mixture. Simply adding primers and template, the reagent will execute primer
extensions and other molecular biology applications. The amaR OnePCR HiFi is a pre-mixed solution containing GDP-HiFi DNA
polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. It contains the fluorescence dye, which is directly
detected on the blue-light transilluminator or UV epi-illuminator after the DNA electrophoresis. The GDP-HiFi DNA polymerase exhibits
excellent processivity, elongation capability, and strong proof-reading activity. The elongation rate of this enzyme is approximately 2 times
higher than that of Taq DNA polymerase. The elongation rate is 106-138 bases/s. and produces blunt end PCR products. The amaR
OnePCR HiFi contents red tracking dyes, provide a safe, non-toxic and non-mutagenic alternative to ethidium bromide for instantaneous
band visualization, that are environmentally friendly containing no hazardous chemicals. The tracking dyes that run at 10 bp on a 1%
agarose gel. The amaR OnePCR HiFi mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume
to be used for the addition of the primer and template solutions. The reagents are provided with sufficient amplification reactions of
20 μl each.

  Features

  • No post-staining procession.
  • No need to prepare PCR reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity- High degree of sensitivity as the ethium bromide.
  • Time Efficiency- No destaining requirement.
  • Compatibility – Use the Blue Light or UV to detect the signal.
  • Effective for the amplification of GC-rich targets.
  • Exhibits strong 3’→5′ exonuclease activityTracking dyes

  Tracking dye

  • Amaranth
Download Protocol
MSDS