amaR OnePCR HiFi

Cat No. SM215-0250 Size: 250 Reactions (2 X 1.25 ml)
Cat No. SM215-0010 Size: 10 Reactions (1 X 100 µl)
Storage
– Store at RT up to 1 months.
– Store at 4°C up to 6 months.
– Store at -20°C up to 1 year.
Note: amaR OnePCR is light sensitive and should be stored and protected from light.
Description

The amaR OnePCR is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer
extensions and other molecular biology applications. The amaR OnePCR is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTPs, gel loading dyes, and fluorescence dye. The amaR OnePCR which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. The amaR OnePCR, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. The amaR OnePCR contents red tracking dyes, provide a safe, non-toxic and non-mutagenic alternative to ethidium bromide for instantaneous band visualization, that are environmentally friendly containing no hazardous chemicals. The tracking dyes that run at 10 bp on a 1% agarose gel. The amaR OnePCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. The reagents are provided with the sufficient amplification reactions of 20 μl each.The amaR OnePCR HiFi is a ready-to-use PCR reaction mixture.

Simply adding primers and template, the reagent will execute primer extensions and other molecular biology applications. The amaR OnePCR HiFi is a pre-mixed solution containing GDP-HiFi DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. It contains the fluorescence dye, which is directly detected on the blue-light transilluminator or UV epi-illuminator after the DNA  lectrophoresis. The GDP-HiFi DNA polymerase exhibits excellent processivity, elongation capability, and strong proof-reading activity. The elongation rate of this enzyme is approximately 2 times higher than that of Taq DNA polymerase. The elongation rate is 106-138 bases/s. and produces blunt end PCR products. The amaR OnePCR HiFi contents red tracking dyes, provide a safe, non-toxic and non-mutagenic alternative to ethidium bromide for instantaneous band visualization, that are environmentally friendly containing no hazardous chemicals. The tracking dyes that run at 10 bp on a 1% agarose gel. The amaR  OnePCR HiFi mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of the primer and template solutions. The reagents are provided with sufficient amplification reactions of 20 μl each.

  Features

  • No post-staining procession.
  • No need to prepare PCR reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity- High degree of sensitivity as the ethium bromide.
  • Time Efficiency- No destaining requirement.
  • Compatibility – Use the Blue Light or UV to detect the signal.
  • Effective for the amplification of GC-rich targets.
  • Exhibits strong 3’→5′ exonuclease activityTracking dyes

  Tracking dye

  • Amaranth
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