| Cat. No. SM317-0250 |
| Size: 50 μl X 5 vial |
| Description The current detection protocol for new coronavirus is mainly nucleic acid detection including: virus sample preparation, viral nucleic acid extraction, RNA reverse transcr iption to cDNA, QPCR detection and analysis. We clone partial gene sequence of Severe acute respiratory syndrome coronavirus 2 (SARSCoV- 2; novel coronavirus (2019-nCoV)), that causes coronavirus disease 2019 into pcDNA3.1(+) vector or pGH vector, respectively. These constructed plasmids could be used as the positive control of 2019-nCoV detection. |
| Quality Control The quality of the 2019-nCoV control plasmid system is tested on a lot-to-lot basis to ensure consistent product quality. |
| Applications ◆ Detection of expressed genes. ◆ Examination of transcript variants. |
| Required Materials ◆ qPCR reagent ◆ Reaction tubes and caps /qPCR plates and seals ◆ qPCR thermal cycling instrument ◆ DEPC- treat water ◆ Gene-specific primers and fluorogenic prob ◆ ROX reference dye (optional) ◆ RNase inhibitor (optional) |
| Plasmid Information | |||
| Plasmid Name | Vector Backbone | Insert Gene Length (bp) | Whole Length (bp) |
| pcDNA-nCoV-S | pCDNA3.1(+) | 3822 | 9188 |
| pcDNA-nCoV-E | pCDNA3.1(+) | 228 | 5619 |
| pcDNA-nCoV-M | pCDNA3.1(+) | 669 | 6060 |
| pcDNA-nCoV-N | pCDNA3.1(+) | 1260 | 6615 |
| pGH-nCoV-1abN | pGH | 739 | 3656 |
| Download | Protocol | SDS | Plasmid Information |
