amaR OnePCR™ HotStar

Cat. No. SM216-0250Size: 250 Reactions (2 X 1.25 ml)
Cat. No. SM216-0005Size: 10 Reactions (1 X 50 µl)
Store at room temperature up to 1 month
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping temperature: 4°C
Note: amaR OnePCR is light sensitive and should be stored and protected from light.
• No post-staining processing of DNA required.
• No need to prepare PCR Reagents.
• Direct loading onto your agarose gel.
• Sensitivity – High degree of sensitivity as the ethidium bromide.
• Time efficiency – No destaining requirement.
• Compatibility –Use the UV light or blue light to detect the signal.
• Specificity – Reduce the non-specific amplification products, thus significantly improving the specificity of PCR reactions
AmaR OnePCR™ HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. AmaR OnePCR™ HotStar is a pre-mixed solution containing hot-start Taq DNA polymerase, PCR reation buffers, dNTPs, gel loading dyes, enhancer, and fluorescence dye. AmaR OnePCR™ HotStar contains hot-start Taq DNA polymerase, which exhibits non-template–dependent terminal transferase activity that adds a 3’ deoxyadenosine to product ends, and 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). The hot-start Taq DNA polymerase is Taq DNA polymerase complexes with a proprietary antibody that blocks activity at room temperatures. When heat to 95°C, the enzyme is restored, providing an automatic “hot start” for Taq DNA polymerase in PCR, increasing the sensitivity and specificity of PCR reaction. AmaR OnePCR™ HotStar contains a red tracking dye, provide a safe, non-toxic and non-mutagenic alternative to Ethidium Bromide for instantaneous band visualization, the dye is environmentally friendly containing no hazardous chemicals. The AmaR OnePCR™ HotStar contains only a single fast-running tracking dye that migrates at approximately 10 base pair in a 1% agarose gel. AmaR OnePCR™ HotStar also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. AmaR OnePCR™ HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with sufficient amplification reactions of 20 μl each.
Tracking Dye
• Amaranth
Quality Control
The quality of the amaR OnePCR™ HotStar is tested on a lot-to-lot basis to ensure consistent product quality.
Required Materials
• Electrophoresis equipments.
• DNA Markers (optional).
• BLooK LED Transilluminator or UV epi-illuminator
Buffer Preparation
• TE buffer, pH8.0 (Selective): 10 mM Tris-HCl, pH 8.0 with 1 mM EDTA