The BlooDireX PCR™ System is designed for amplification of DNA from whole blood. It contains BlooDireX PCR and Novel Juice, suitable for PCR from blood sample directly. BlooDireX PCR™ is performed PCR directly from sample with no prior DNA extraction or sample preparation. For blood, stored at 4 °C or frozen, and preserved with EDTA, citrate or heparin are all suitable for this master mix. BlooDireX PCR™ is a premixed ready- to-use solution containing reaction buffers, dNTPs, loading dye, glycerol, PCR enhancers, and hot- start DNA polymerase. The hot-start taq prevents non-specific amplification due to mispriming and/or formation of primer dimers before thermal cycling, and it also exhibits 2X faster elongation rate than conventional Taq. The modified hot-start DNA polymerase is resistant to most PCR inhibitors and retains its activity in the PCR reaction. BlooDireX PCR™ is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 20 μl each. Novel Juice is a
non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon blue light (e.g. BLooK, BK001) or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Novel Juice is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels. Novel Juice is the most sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis process. It is ideal for the environment requiring a safe, non-hazardous alternative to Ethidium Bromide (EtBr). Approximate fluorescence excitation / emission: 300, 495 / 537 nm, bound to nucleic acid.