GeneDireX, Inc.

We provide the following custom service. You are welcome to contact us for more information by e-mailing to info@genedirex.com

Dual Labeled Probe

We provide custom designed dual labeled probe set, including one probe and a pair of primers.

➤ Regular package: one vial of probe 5 OD (HPLC) with 5’FAM (reporter) and 3’ TAMRA (quencher), and two vials of primers 2 OD each (PAGE).

➤ No extra charge for probe and primer design.

➤ BHQ: black hole quencher, one kind of nonfluorescent quencher.

FluorescenceExcitation maximum (nm)Emission maximum (nm)
FAM490525
JOE520548
Cy3554568
Cy5649666
ROX575602
QuencherAbsorption Max, nmQuenching Range, nm
TAMRA544520-570
BHQ1534480-580
BHQ2579550-650
BHQ3672620-730
5'-FAM/3'-BHQ15'-FAM/3'-TAMRA
5'-JOE/3'-BHQ15'-JOE/3'-TAMRA
5'-ROX/3'-BHQ25' -Cy3/3'-BHQ2
5' -Cy5/3'-BHQ3

PRIME Gene Synthesis & Subcloning

Custom Gene Synthesis Service — From DNA sequences to constructs with 100% accuracy guaranteed.

➤ We provide gene of interest into shuttle vector (pUC57, or other) of 2~4 μg lyophilized plasmid, sequencing data and QC reports

➤ Subcloning service: we can subclone the target gene sequence into your choice expression vector.

➤ We provide codon optimization for better protein yield.

➤ We provide library construction service including combinatorial variant libraries, CRISPR library, and NGS library probes.

Custom Peptide Synthesis

➤ We can produce peptides from 3 to 100 AAs in length at purities from 80% up to 98%.

➤ Peptide quantity: 1~4 mg, 5~9 mg, 10 mg, 15 mg, 20 mg and other quantities.

➤ Research and cosmetic grade

➤ Modifications:

Fluorescent labelingBiotin conjugationAhx linker or long carbon (LC) linkerBSA, KLH and OVA conjugation
FRET pairsD formAmidation and acetylationPhosphorylation
MethylationDisulfide bridgeSide chain modificationCyclization

➤ custom peptide library service for screening including overlapping peptide library, alanine scanning library, truncation library, positional scanning library, random library and scrambled library.

➤ QC data: MS and HPLC analysis for each peptide

➤ Peptide solubility test

➤ Optional service:

  • Endotoxin test
  • TFA removal (salt form: acetate and hydrochloride)
  • Aliquoting service
    •  

Custom Antibody Production Service

We offer high quality custom antibody production services and professional antigen design. We provide professional affinity purification technique to increase the purity of antibodies.

Custom Rabbit Polyclonal Antibody

Reports and products

1. Control IgG antibody, 50 μg
2. Purified rabbit polyclonal antibody, 2-10 mg
3. QC report of antibody titration by ELISA , titer > 1:50,000

Custom Mouse Monoclonal Antibody

Reports and products

1. Two cryo-vials of hybridoma clones.
2. Each 5 mL supernatant from hybridoma culture media for each hybridoma clone.
3. QC report of antibody titration by ELISA

Acro miR Vector System

MicroRNA are typically 19 to 22 nucleotides long and are thought to regulate gene expression posttranscriptionally by binding to the 3 ́ untranslated regions (UTRs) of target mRNAs and inhibiting their translation.

For constitutive microRNA expression, Acro miR vector construction is the best choice.
● We provide pre-miR, miR and miR inhibitor vector construction service.
● Latest version of miRBbase sequence database version 22.1.
● Deliverable: One vial of 50ug for one miR vector construct.
● Delivery form: lyophilizing DNA powder

Acro miR mimics & inhibitor System

➤ We provide updated and comprehensive miR mimics and inhibitors for human, mouse, rat, and other species.
➤ Latest version of miRBbase sequence database version 22.1.
➤ Regular package: One vial of 4 OD for double-stranded miR mimics or single-stranded miR inhibitors plus 1 OD of FAM-labeled universal negative control     for free.
➤ High quality: HPLC purification.
➤ 2’OMe modification provides high stability.
➤ Modification:biotin, fluorescence-conjugation (FAM, Cy3, and Cy5) linker (5’-NH2 or 3’-NH2) and special labeling are available
➤ Special-modified Angomir and Antagomir are also available.
  • 2’OMe modification
  • Phosphorothioate oligonucleotides
  • Phosphorylation
  • 3’ Cholesterol
  • 5’ fluorescence conjugation is available.

Acro shRNA System

RNA interference (RNAi) is the most effective method to knock down gene expression with transfected siRNA. shRNAs are used in vector-based approaches for supplying siRNA to cells to produce stable gene silencing. We provide custom and pre-designed shRNA construct.

  • Regular package: One vial of 50ug for one shRNA construct
  • Regular set: Four vials of 50ug for each shRNA constructs of target gene plus one negative control for free.
shRNA setTarget geneUniversal N.CPositive Control (GAPDH or β-actin)Amount of shRNA
pGP system4116
pGLV system41NA5
  • Comprehensive vectors are available, including GFP, RFP and mCherry reporters and lentiviral backbone.
  • Constitutive promoters are available (U6/H1).
  • We provide custom shRNA design and siRNA to shRNA design.

Acro siRNA System

RNA interference (RNAi) is the most effective method to knock down gene expression to study protein function. For transient silencing experiments, siRNAs are the best choice for this purpose.

  • We provide custom and pre-designed siRNA duplex.
  • Regular package: One vial of 4 OD for siRNA duplex plus 1 OD of FAM-labeled universal negative control for free.
  • Regular set: Three vials of 2 OD for each siRNA duplex plus 1 OD of FAM-labeled universal negative control for free.
  • High quality: HPLC purification.
  • 2’OMe modification prolong siRNA half-life and provides high stability.
  • Modification: Biotin, fluorescence-conjugation(FAM, Cy5, and Cy3), linker(5’-NH2 or 3’-NH2) and special labeling are available.
  • Special custom modification is available.
  • We provide siRNA design.