We provide the following custom service. You are welcome to contact us for more information by e-mailing to info@genedirex.com
Dual Labeled Probe
➤ We provide custom designed dual labeled probe set, including one probe and a pair of primers.
➤ Regular package: one vial of probe 5 OD (HPLC) with 5’FAM (reporter) and 3’ TAMRA (quencher), and two vials of primers 2 OD each (PAGE).
➤ No extra charge for probe and primer design.
➤ BHQ: black hole quencher, one kind of nonfluorescent quencher.
Fluorescence | Excitation maximum (nm) | Emission maximum (nm) |
---|---|---|
FAM | 490 | 525 |
JOE | 520 | 548 |
Cy3 | 554 | 568 |
Cy5 | 649 | 666 |
ROX | 575 | 602 |
Quencher | Absorption Max, nm | Quenching Range, nm |
---|---|---|
TAMRA | 544 | 520-570 |
BHQ1 | 534 | 480-580 |
BHQ2 | 579 | 550-650 |
BHQ3 | 672 | 620-730 |
5'-FAM/3'-BHQ1 | 5'-FAM/3'-TAMRA |
5'-JOE/3'-BHQ1 | 5'-JOE/3'-TAMRA |
5'-ROX/3'-BHQ2 | 5' -Cy3/3'-BHQ2 |
5' -Cy5/3'-BHQ3 |
PRIME Gene Synthesis & Subcloning
Custom Gene Synthesis Service — From DNA sequences to constructs with 100% accuracy guaranteed.
➤ We provide gene of interest into shuttle vector (pUC57, or other) of 2~4 μg lyophilized plasmid, sequencing data and QC reports
➤ Subcloning service: we can subclone the target gene sequence into your choice expression vector.
➤ We provide codon optimization for better protein yield.
➤ We provide library construction service including combinatorial variant libraries, CRISPR library, and NGS library probes.
Custom Peptide Synthesis
➤ We can produce peptides from 3 to 100 AAs in length at purities from 80% up to 98%.
➤ Peptide quantity: 1~4 mg, 5~9 mg, 10 mg, 15 mg, 20 mg and other quantities.
➤ Research and cosmetic grade
➤ Modifications:
Fluorescent labeling | Biotin conjugation | Ahx linker or long carbon (LC) linker | BSA, KLH and OVA conjugation |
FRET pairs | D form | Amidation and acetylation | Phosphorylation |
Methylation | Disulfide bridge | Side chain modification | Cyclization |
➤ custom peptide library service for screening including overlapping peptide library, alanine scanning library, truncation library, positional scanning library, random library and scrambled library.
➤ QC data: MS and HPLC analysis for each peptide
➤ Peptide solubility test
➤ Optional service:
- Endotoxin test
- TFA removal (salt form: acetate and hydrochloride)
- Aliquoting service
Custom Antibody Production Service
We offer high quality custom antibody production services and professional antigen design. We provide professional affinity purification technique to increase the purity of antibodies.
Custom Rabbit Polyclonal Antibody
Reports and products
1. Control IgG antibody, 50 μg
2. Purified rabbit polyclonal antibody, 2-10 mg
3. QC report of antibody titration by ELISA , titer > 1:50,000
Custom Mouse Monoclonal Antibody
Reports and products
1. Two cryo-vials of hybridoma clones.
2. Each 5 mL supernatant from hybridoma culture media for each hybridoma clone.
3. QC report of antibody titration by ELISA
Acro miR Vector System
MicroRNA are typically 19 to 22 nucleotides long and are thought to regulate gene expression posttranscriptionally by binding to the 3 ́ untranslated regions (UTRs) of target mRNAs and inhibiting their translation.
● Deliverable: One vial of 50ug for one miR vector construct.
● Delivery form: lyophilizing DNA powder
Acro miR mimics & inhibitor System
- 2’OMe modification
- Phosphorothioate oligonucleotides
- Phosphorylation
- 3’ Cholesterol
- 5’ fluorescence conjugation is available.
Acro shRNA System
RNA interference (RNAi) is the most effective method to knock down gene expression with transfected siRNA. shRNAs are used in vector-based approaches for supplying siRNA to cells to produce stable gene silencing. We provide custom and pre-designed shRNA construct.
- Regular package: One vial of 50ug for one shRNA construct
- Regular set: Four vials of 50ug for each shRNA constructs of target gene plus one negative control for free.
shRNA set | Target gene | Universal N.C | Positive Control (GAPDH or β-actin) | Amount of shRNA |
pGP system | 4 | 1 | 1 | 6 |
pGLV system | 4 | 1 | NA | 5 |
- Comprehensive vectors are available, including GFP, RFP and mCherry reporters and lentiviral backbone.
- Constitutive promoters are available (U6/H1).
- We provide custom shRNA design and siRNA to shRNA design.
Acro siRNA System
RNA interference (RNAi) is the most effective method to knock down gene expression to study protein function. For transient silencing experiments, siRNAs are the best choice for this purpose.
- We provide custom and pre-designed siRNA duplex.
- Regular package: One vial of 4 OD for siRNA duplex plus 1 OD of FAM-labeled universal negative control for free.
- Regular set: Three vials of 2 OD for each siRNA duplex plus 1 OD of FAM-labeled universal negative control for free.
- High quality: HPLC purification.
- 2’OMe modification prolong siRNA half-life and provides high stability.
- Modification: Biotin, fluorescence-conjugation(FAM, Cy5, and Cy3), linker(5’-NH2 or 3’-NH2) and special labeling are available.
- Special custom modification is available.
- We provide siRNA design.