|Cat No. MB601-0100||Size: 100 Reactions|
GDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). GDP-HiFi has higher stability at high temperature. Users may program the initial denaturizing at even 100°C for 2 min, and this property makes GDP-HiFi perform very well for GC-rich PCR.
This special enzyme has been modified genetically and needs less concentration of magnesium than other polymerases. The suggestion for magnesium ion in the reaction is 0.8 to 1.2 mM. 10X GDP-HiFi PCR buffer contains no magnesium. A tube of 25-mM MgSO4 is provided. Further optimization can be achieved for different targets of DNAs.
Reagents are provided for 100 PCRs of 50 μl each.