
Cat. No. MB305-0050 | Size: 50 Reactions |
Cat. No. SM305-0050 | Size: 50 Reactions |
Description The GScript First-Strand Synthesis Kit is a recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has a lower RNase H activity and a high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb. The GScript RTase has a special activity with its secondary structure, rendering stabilization during the reverse transcription reaction. |
Quality Control The quality of the GScript First-Strand Synthesis Kit is tested on a lot-to-lot basis to ensure consistent product quality. |
Download | |
MB305-0050 | SM305-0050 |
Protocol | Protocol |
MSDS | |
MB305-0050 | SM305-0050 |
GScript RTase | GScript RTase |
5X 1st strand buffer | 5X 1st strand buffer |
DTT | DTT |
dNTP mix | dNTP mix |
Oligo(dT)20 | Oligo(dT)20 |