| Cat No. MB305-0050 | Size: 50 Reactions |
| Description The GScript First-Strand Synthesis Kit is a recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has a lower RNase H activity and a high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb. The GScript RTase has a special activity with its secondary structure, rendering stabilization during the reverse transcription reaction. |
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| Kit Contents | |
| Component | Volume (μL) |
| Oligo(dT)20(50 μM) | 50 |
| 5X 1st strand buffer | 250 |
| DTT (0.1 M) | 100 |
| dNTP mix (10 mM) | 50 |
| GScript RTase (200 U/ μl) | 50 |
| Quality Control The quality of the GScript First-Strand Synthesis Kit is tested on a lot-to-lot basis to ensure consistent product quality. |
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Protocol |
| MSDS |
