The GScript One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps. The GScript One-Step RT-PCR Kit consists of two major components: GScript Enzyme Mix and 5X Reaction Mix, provided a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains enhancer that optimizes the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this GScript One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional. The GScript Enzyme Mix is for optimal cDNA synthesis and PCR amplification. The enzyme mix uses a mixture of M-MLV Reverse Transcriptase and Hotstart Taq DNA polymerase in an optimized reaction buffer.The 5X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. This convenient 5X format allows further addition of template and primer at any desired concentration. Sufficient reagents are provided for 50 amplification reactions of 50 μl each.