|Cat. No. MB303-0050||Size: 50 Reactions|
|Cat. No. SM303-0050||Size: 50 Reactions|
The GScript RTase is recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has lower RNase H activity and high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb.
|First-Strand cDNA Synthesis
1. In a sterile microfuge tube, first add:
2. Heat for 3-5 minutes at 65°C. Spin briefly and place promptly on ice.
If generating cDNA longer than 5 kb at temperatures above 50°C using a gene-specific primer or oligo(dT)20, the amount of GScript RTase may be raised to 400 U (2 μl) to increase yield.
Store all components at -20°C (non-frost-free).
Thaw 5X RT Buffer, 0.1 M DTT at room temperature justprior to use and refreeze immediately.