GScriptULTRA First-Strand Synthesis Kit (Random primer)

Cat. No. SM310-H100
Size: 100 Reactions
Store at -20°C
◆ Time efficiency – included most reagents for reverse transcription reaction.
The GScriptULTRA First-Strand Synthesis Kit (Random primer) provides a convenient and sensitive of cDNA synthesis from RNA molecules by reverse transcription (RT). The kit consists of five major components: GScriptULTRA RTase, 5X 1st strand buffer, 0.1 M DTT, 50 μM Random Hexamers primer, and 10 mM dNTP (Deoxyribonucleotide triphosphates) mix. The GScriptULTRA RTase is a recombinant Moloney Murine Leukemia Virus (M-MLV) transcription polymerase expressed in E. coli and purified to homogeneity. It has lower RNase H activity and high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 base pairs (bps) to 12 Kilo base pairs (kb). The 5X Reaction Mix buffer is optimized for reverse transcription. The DTT breaks the disulfide bonds, loosen the secondary structure of RNA, and helps in initiation for cDNA synthesis. Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target RNA. The dNTP solution consists all four nucleotides (dATP, dCTP, dGTP, dTTP), suitable for use in cDNA synthesis.
Quality Control
The quality of the GScriptULTRA First-Strand Synthesis Kit (Random primer) is tested on a lot-to-lot basis to ensure consistent product quality.
ProtocolRandom Hexamers (50 μM)
5X 1st strand buffer
DTT (0.1 M)
dNTP mix (10 mM)
GScriptULTRA RTase