The Novel Express qPCR System-ROX Free provides a convenient, simple, rapid, high sensitivity, specificity, stability, and robust set-up for performing quantitative real-time analysis of DNA samples. The kit is with the proprietary concentration with the HotStart DNA polymerase、SYBR Green I、dNTPs、Mg2+、 reaction buffer, and enhance stabilizer. The HotStart DNA polymerase is inactive under the 75°C that avoid the non-specific hybrid or primer dimer at the room temperature. The activation of the reverse transcriptase enzyme is under 95°C for 10 min and the HotStart DNA polymerase will start running, so users can operating the mixture of reaction buffer can under the bench.
The SYBR Green I fluorescent dye was specific binding with the DNA double strands ensure the consistency of the fluorescent singal and PCR Product. Novel Express qPCR System-ROX Free is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer, template solutions, and RNase– free H2O. Reagents are provided with the sufficient amplification reactions of 20 μl each.