Novel Express qPCR System-ROX I

Cat. No.: QP001-0100 Size: 100 Reactions
Cat. No.: QP001-0020 Size: 20 Reactions
Sample: 10~100 ng DNA or 1~10 ng cDNA
Store at -20°C up to 1 year and protected from light.
Description
The Novel Express qPCR System-ROX I provides a simple, specificity, and stability for performing quantitative analysis of DNA samples. The kit has the proprietary concentration with the HotStart DNA polymerase, SYBR Green I, dNTPs, Mg2+, reaction buffers, enhance stabilizer, and ROX I reference dye. The Novel Express qPCR System-ROX I contains a hot-start DNA polymerase that blocks activity at room temperatures. Activation is restored after the enzyme activation step at 95°C, thereby providing an automatic “hot start” for Taq DNA polymerase in real-time PCR (qPCR). The SYBR Green I fluorescent dye is specific binding with the DNA double strands ensure the consistency of the fluorescent signal and qPCR product. The ROX I reference dye includes in the reagent provides an internal reference to the reporter dye, the signal can be normalized, which is necessary to correct for fluorescent fluctuations due to changes in concentration, volume, or light source intensity. The normalization of the reporter dye signal results in increased data precision. The Novel Express qPCR System-ROX I is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer, template solutions, ROX I reference Dye (Depending on the use of fluorescent qPCR instrument, check on Compatible Instruments part), and Nuclease-free H2O. Reagents are provided with the sufficient amplification reactions of 20 μl each.
Kit contents
Contents QP001-0100 QP001-0100
Novel Express qPCR Reagent 1000 μl 200 μl
ROX I Reference Dye (50X) 40 μl 8 μl
Protocol
MSDS