One-Step qRT-PCR

  • Convenience -Both cDNA synthesis and PCR are performed in a single tube
  • Specificity –Formulated for use with fluorogenic probe-based technology
  • Flexible –Compatible with standard or fast mode program
Cat. No. MB307-0100 Size: 100 Reactions
Description
The One-Step qRT-PCR Kit are compatible with TaqMan probes. The Kit provides a convenient, sensitive, and reproducible method to detect and quantify the RNA molecules by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The components for cDNA synthesis, PCR amplification, and quantification are combined in a kit, using gene-specific primers, probes, and target RNAs from either total RNA or mRNA. The kit consists of three major components: RT/ HotStar Taq Mix, 2X Reaction Mix, and 50 mM Magnesium Sulfate (MgSO4). The RT/ HotStar Taq Mix contain a mixture of Reverse Transcriptase (RTase) and HotStar Taq DNA polymerase for optimal cDNA synthesis and PCR amplification. The RTase is modified from the Moloney Murine Leukemia Virus (M-MLV) RTase, engineered to reduce RNase H activity and increase thermal stability. The HotStar Taq DNA polymerase is Taq DNA polymerase complexes with a proprietary antibody that blocks activity at ambient temperatures. Activity is restored after the enzyme activation step at 95°C, thereby providing an automatic “hot-start” for Taq DNA polymerase in PCR, increasing the sensitivity and specificity of PCR reaction. The 2X Reaction Mix consists of 6 mM MgSO4, deoxyribonucleotide triphosphates (dNTPs), and stabilizers provide a proprietary buffer system optimized for reverse transcription and PCR amplification. One addition tube of 50 mM MgSO4 is included in the kit. The 2X Reaction Mix is well for most targets; however, the optimal concentration may range from 3 to 6 mM. If necessary, use the separate 50 mM MgSO4 buffer to increase the magnesium concentration.
Feature

  • Time efficiency – included most reagents for qRT-PCR reaction.
Applications

  • Detection of expressed genes.
  • Examination of transcript variants.
Quality Control
The quality of the One-Step qRT-PCR Kit is tested on a lot-to-lot basis to ensure consistent product quality.
Download
SM307-0100 Protocol MSDS