OnePCR HotStar

Cat. No. MB206-0100 Size: 100 Reactions ( 2 × 1.25 ml )
Cat. No. SM206-0100 Size: 100 Reactions ( 2 × 1.25 ml )
Description
OnePCR™ HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ HotStar is a pre-mixed solution containing Hot Start Taq DNA polymerase, PCR reation buffers, dNTPs, gel loading dyes, enhancer, and fluorescence dye. OnePCR™ HotStar contains Hot Start Taq DNA polymerasewhich exhibits non-template-dependent terminal transferase activity that adds a 3’ deoxyadenosine to product ends, and 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). The enzyme contains a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the initial denaturation step in PCR cycling at 94°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR. OnePCR™ HotStar also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. OnePCR™ HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with sufficient amplification reactions of 50 μl each.
Application

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel.
  • Sensitivity – High degree of sensitivity as the ethidium bromide.
  • Time efficiency – No destaining requirement.
  • Compatibility –Use the UV light or blue light to detect the signal.
  • Specificity – Reduce the non-specific amplification products, thus significantly improving the specificity of PCR reactions
Storage
Store at room temperature up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping temperature: 4°C
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MB206-0100 Protocol
MSDS
SM206-0100 Protocol
MSDS