OnePCR™

Cat. No. MB203-0100 Size: 100 Reactions
Cat. No. SM203-0100Size: 100 Reactions
Storage
Store at RT up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping Temperature: 4°C
Note: OnePCR™ is light sensitive and should be stored and protected from light.

Description
OnePCR™ is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.OnePCR™ which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR™, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR™ mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.Reagents are provided with the sufficient amplification reactions of 50 μl each.
Features
◆  No post-staining processing of DNA required.
◆  No need to prepare PCR Reagents.
◆  Direct loading onto your agarose gel for analysis.
◆  Sensitivity – High degree of sensitivity as the ethium bromide.
◆  Speed – No destaining requirement.
◆  Compatibility – Use the blue light or UV to detect the signal.
Fig. 1

Fig.1 OnePCR products are viewed using the Blue Light (left) or UV (right) to detect the signal.
Exposure to the Blue Light (left gel), and UV light (right gel)
Lane M: 1 kb Marker
Lanes 1~3: 1 kb, lanes 4~6: 2 kb, lanes 7~9: 4 kb
Template: p35s-GFP

Fig. 2

Fig. 2 PCR products of the genomic DNA (lane 1) or cDNA (lane 2) of Rose petal.
Exposure to the Blue Light.
Primer: Actin Forward Primer / Actin Reverse Primer
Lane M: 1 kb Marker

Fig. 3

Fig. 3 PCR products of the storage condition test for 3 months.
Exposure to the Blue Light.
M: 1 kb Marker
Lane 1: Room temperature for 3 months
Lane 2: 4°C for 3 months
Lane 3: -20°C for 3 months
Lane 4: 0 day

FIg. 4

Fig. 4 Sequence analysis . PCR products of both the general PCR(up) and OnePCR(down) from the p35s-GFP plasmid sequencing results exhibited as the same.

Fig. 5

Fig. 5 Results of the colony PCR separation on the 1.2% agarose gel.
Exposure to the UV light
Lane M: 1 kb Marker
Target: Lanes 1~3 : 1kb
Lanes 4~6: 4kb
Lanes 7~ 9 : 2kb
Template: p35S-GFP Plasmid

Fig. 6

Fig. 6 Cloning test of the OnePCR and general PCR products.After ligation , perform the PCR check on the insert.
Exposure to the Blue Light.
Insert: General PCR and OnePCR reaction products .
Primer : M13 Forward Primer / M13 Reverse Primer
Lane M: 1 kb Marker
Lane 1: General PCR
Lane 2: OnePCR


Application  
◆  No post-staining processing of DNA required.
◆  No need to prepare PCR Reagents.
◆  Direct loading onto your agarose gel for analysis.
◆  Sensitivity – High degree of sensitivity as the ethium bromide.
◆  Speed – No de-staining requirement.
◆  Compatibility – Use the blue light or UV to detect the signal.
Mammalian Chromosomal Aberration Test
Mutation Test Report
Acute Toxicity Report
Download
MB203-0100ProtocolMSDS
SM203-0100 ProtocolMSDS