| Cat. No. PM005-0500 | Size: 500 μl |
| Cat. No. SP005-0500 | Size: 250 μl x 2 |
| Features ◆ Broad range: 10-175 kDa (Tris-glycine-SDS running buffer) ◆ Ready-to-use: supplied in a loading buffer for direct loading on gels ◆ Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dyes ◆ Sharp bands |
| Description The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kilodalton (kDa). The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. |
| Applications ◆ Monitoring of protein migration during SDS-PAGE gel electrophoresis. ◆ Monitoring of protein transfer onto membranes during Western blots. ◆ Sizing of proteins on SDS-PAGE gels and Western blots. |
| Storage Buffer Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol. |
| Quality Control The quality of the PiNK Plus Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality. |
| Storage Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C. |
| PiNK Plus Prestained Protein Ladder Protocol 1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil. 2. Mix thoroughly to ensure the solution is homogeneous. 3. Load the following volumes of the ladder on SDS-PAGE gel: ◆ 5 μl per well for mini-gels, 2.5 μl per well for blots ◆ 10 μl per well for large gels, 5 μl per well for blots ◆ Apply more for thicker (> 1.5 mm) or larger gel |
| Guide for Molecular Weight Estimation (kDa) Migration patterns of PiNK Plus Prestained Protein Ladder in different electrophoresis conditions are listed below: |
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