|Cat No. PM005-0500||Size: 500 μl|
|Cat No. SP005-0600||Size: 600 μl|
The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol).
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
For long term storage, store at -20°C.
|Recommendations for Loading
1.Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly to ensure the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
– 5 μl per well for mini-gels, 2.5 μl per well for blots
– 10 μl per well for large gels, 5 μl per well for blots