|Cat. No. PM005-0500||Size: 500 μl|
|Cat. No. SP005-0500||Size: 250 μl x 2|
◆ Broad range: 10-175 kDa (Tris-glycine-SDS running buffer)
◆ Ready-to-use: supplied in a loading buffer for direct loading on gels
◆ Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dyes
◆ Sharp bands
The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kilodalton (kDa). The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
◆ Monitoring of protein migration during SDS-PAGE gel electrophoresis.
◆ Monitoring of protein transfer onto membranes during Western blots.
◆ Sizing of proteins on SDS-PAGE gels and Western blots.
Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol.
The quality of the PiNK Plus Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
For long term storage, store at -20°C.
|PiNK Plus Prestained Protein Ladder Protocol|
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-PAGE gel:
◆ 5 μl per well for mini-gels, 2.5 μl per well for blots
◆ 10 μl per well for large gels, 5 μl per well for blots
◆ Apply more for thicker (> 1.5 mm) or larger gel
|Guide for Molecular Weight Estimation (kDa)|
Migration patterns of PiNK Plus Prestained Protein Ladder in different electrophoresis conditions are listed below: