Plasmid midi PREP Kit

Cat No. NA205-0020 Size: 20 Reactions
Cat No. SN205-0020 Size: 20 Reactions
Description
The Plasmid midiPREP Kit provides a fast, simple, and cost-effective method for the plasmid DNA isolation from the cultured bacterial cells. The Plasmid midiPREP Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid midiPREP Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.
Features

  • Ready-to-use DNA for high performance in any downstream application.
  • Consistent DNA yields from a small amount of the starting material.
  • Time flexibility.

 

Protocol
MSDS