2x PCR mix is a ready-to-use PCR reaction mixture. Simply add primers, template, the reagent will execute primer extensions and other molecular biology applications. 2x PCR mix is a pre-mixed solution containing Taq DNA polymerase, PCR reaction buffers, and dNTPs. 2x PCR mix contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. 2x PCR mix mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addit ion of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.
- PCR Amplification
The quality of the 2x PCR mix is tested on a lot-to-lot basis to ensure consistent product quality.