The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.
MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRwith polyhistidine tag at the C-terminus.
polyhistidine tag at the C-terminus
<0.1 EU per 1 μg of the protein by the LAL method.
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.
The ED₅₀ for this effect is <0.58 ng/mL.
>95% as determined by SDS-PAGE. Ni-NTA chromatography
The protein was lyophilized from a solution containing 20 mM sodium carbonate, pH 9.0.
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.
SDS-PAGE analysis of recombinant human BMP-4