GeneDireX, Inc.

Category

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ECLong

Size: 250mL x 2


Features

  • Signal Duration: 12 hours
  • Detection Method: X-ray film or imaging acquisition system
  • Suggested Antibody Dilution:
    Primary: 1/1,000 – 1/50,000
    Secondary: 1/50,000 – 1/250,000
  • Lower Detection Limit:
    Low-Picogram (10⁻¹⁴)
    High-Zeptomole (10⁻¹⁹)

NT$6,000

Cat.No. SM801-0500 Categories ,

The principle of ECLong is based on chemiluminescence and is very convenient to detect the Horseradish peroxidase (HRP) activity in many assays such as Western blotting, Southern blotting, and Northern blotting. HRP catalyzes the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol by
hydrogen peroxide (H₂O₂). ECLong can enhance the luminol-dependent chemiluminescence and be wildly used to detect the present of HRP-conjugated antibodies or streptavidin which binding to antigen or nucleotide sequence respectively.


Application

  • Western blotting

Quality Control
The quality of the ECLong is tested on a lot-to-lot basis to ensure consistent product quality.

Protocol

SM801-0500

SDS

SM801-0500A_MSDS

SM801-0500B_MSDS

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