GeneDireX, Inc.

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Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Human

GMP Grade Available!


Size: 100 μg


Storage
Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.


Note
Please use within one month after protein reconstitution.

Cat.No. SR215-0100 Categories ,

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells, and fibroblasts that functions as a cytokine.GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by the reproductive tract.


Expression System
Escherichia coli


Sequence
APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE with polyhistidine tag at the N-terminus.


Species
Human


Tag
polyhistidine tag at the N-terminus


Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.


Activity
Measure by its ability to induce TF-1 cells proliferation.
The ED50 for this effect is <80 pg/mL.
The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.


Purity
>98% as determined by SDS-PAGE. Ni-NTA chromatography


Formulation
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.


Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.


SDS-PAGE analysis of recombinant human GM-CSF

Protocol

SR215-0100

SR215-0100G

Document Searching (Protocol, CoA, SDS)

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