The GScript One-Step RT-qPCR Mix has been freeze-dried to produce a room temperature stable preparation. The master mix is designed for a fast and easy one-step assay that combines reverse transcription and real-time PCR reactions. The lyophilized powder contains reverse transcriptase, hot-start Taq DNA Polymerase as well as dNTPs and buffer components at optimal concentrations, and it can be equipped with 2X pre-mix quickly and conveniently. This product is compatible with TaqMan probes. The reverse transcriptase is modified from the Moloney Murine Leukemia Virus (M-MLV) RTase that can tolerate high temperature of 60°C, which is helpful for reverse transcription of RNA templates with complex secondary structures. The hot-start Taq DNA polymerase is resistant to heparin, humic acid and high salt concentrations, and is more suitable for RNA detection in blood and tissue samples.
The quality of the GScript One-Step RT-qPCR Mix is tested on a lot-to-lot basis to ensure consistent product quality.
Shipping at ambient temperature and remaining stable quality as non-lyophilized formulation