GeneDireX, Inc.

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GScript One-Step RT-qPCR Mix, lyophilized

Size: 50 Reactions

Features

  • Lyophilized form – room temperature stable transportation.
  • Time efficiency – included most reagents for RT-qPCR reaction.
  • Use reverse transcriptase that can tolerate 60°C.
  • Use hot-start Taq DNA polymerase that can tolerate heparin, humic acid and high salt.

Storage
Store at room temperature on arrival and stable at 4°C up to the expiry date.
Once re-suspended with the Nuclease-free water, the master mix should be stored at -20°C up to the expiry date stated. Keep in aliquot to reduce freeze-thaw cycles.

NT$99,999

Cat.No. SM308-0050 Categories , Tag

The GScript One-Step RT-qPCR Mix has been freeze-dried to produce a room temperature stable preparation. The master mix is designed for a fast and easy one-step assay that combines reverse transcription and real-time PCR reactions. The lyophilized powder contains reverse transcriptase, hot-start Taq DNA Polymerase as well as dNTPs and buffer components at optimal concentrations, and it can be equipped with 2X pre-mix quickly and conveniently. This product is compatible with TaqMan probes. The reverse transcriptase is modified from the Moloney Murine Leukemia Virus (M-MLV) RTase that can tolerate high temperature of 60°C, which is helpful for reverse transcription of RNA templates with complex secondary structures. The hot-start Taq DNA polymerase is resistant to heparin, humic acid and high salt concentrations, and is more suitable for RNA detection in blood and tissue samples.

Quality Control
The quality of the GScript One-Step RT-qPCR Mix is tested on a lot-to-lot basis to ensure consistent product quality.

Shipping at ambient temperature and remaining stable quality as non-lyophilized formulation

Protocol

SM308-0050

MSDS

SM308-0050_MSDS

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