GeneDireX, Inc.

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GScriptULTRA First-Strand Synthesis Kit (Oligo-dT)

Size: 100 Reactions


Store at -20°C
Minimum shelf life: 1 year


Feature

  • Time efficiency – included most reagents for reverse transcription reaction.

Kit contents

Contents Size
Oligo(dT)₂₀ (50 μM) 100 μl
5X 1st strand buffer 500 μl
DTT (0.1 M) 100 μl
dNTP mix (10 mM) 100 μl
GScriptULTRA RTase 40 μl

Cat.No. MB310-T100 Categories ,

The GScriptULTRA First-Strand Synthesis Kit (Oligo-dT) provides a convenient and sensitive of cDNA synthesis from RNA molecules by reverse transcription (RT). The kit consists of five major components: GScriptULTRA RTase, 5X 1st strand buffer, 0.1 M DTT, 50 μM Oligo(dT)₂₀, and Random Hexamers primer, and 10 mM dNTP (Deoxyribonucleotide triphosphates) mix. The GScriptULTRA RTase is a recombinant Moloney Murine Leukemia Virus (M-MLV) transcription polymerase expressed in E. coli and purified to homogeneity. It has lower RNase H activity and high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 base pairs (bps) to 12 Kilo base pairs (kb). The 5X Reaction Mix buffer is optimized for reverse transcription. The DTT breaks the disulfide bonds, loosen the secondary structure of RNA, and helps in initiation for cDNA synthesis. The Oligo(dT)₂₀ consist of a stretch of 20 deoxythymidines that anneal to poly(A) tails of
eukaryotic mRNAs. The primers are optimized for constructing cDNA libraries. The dNTP solution consists all four nucleotides (dATP, dCTP, dGTP, dTTP), suitable for use in cDNA synthesis.


Application

  • Downstream application for PCR to detection of expressed genes, examination of transcript variants, or generation of cDNA templates for cloning and sequencing.

Quality Control
The quality of the GScriptULTRA First-Strand Synthesis Kit (Oligo-dT) is tested on a lot-to-lot basis to ensure consistent product quality.

Protocol

MB310-T100

MSDS

Oligo(dT)₂₀ (50 μM)

5X 1st strand buffer

DTT (0.1 M)

dNTP mix (10 mM)

GScriptULTRA RTase

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