Interleukin-1 Beta (IL-1 Beta) is produced by activated macrophages, IL-1 Beta stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 Beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin from synovial cells.
MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus.
polyhistidine tag at the C-terminus
<0.1 EU per 1 μg of the protein by the LAL method.
Measure by its ability to induce proliferation in D10.G4.1 cells.
The ED50 for this effect is <10 pg/mL.
The specific activity of recombinant human IL-1 beta is approximately >1.5 x 10⁸ IU/mg.
Measure by its ability to induce IL-8 secretion in HT29 cells.
The ED50 for this effect is 1.8-5.1 ng/mL.
>98% as determined by SDS-PAGE. Ni-NTA chromatography
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
Unopened ampoules can be stored at -20°C or -80°C.
Need not spin the ampoule. Open the cap carefully, then dissolve the lyophilized protein with sterile water for a 100 μg/mL concentration or according to the product’s Certificate of Analysis (CoA). Rinse the inner side of the ampoule gently and stand for at least 20 minutes at room temperature. (Important Note: Do Not Vortex!)
Use the reconstituted solution immediately or store it by distributing it to aliquots and store at -20 °C. (Avoid repeated freeze-thaw cycles) We recommend diluting the solution to working concentration by using buffers or medium containing a carrier*. (Do not dilute the working solution with water, which may cause protein degradation.)
*Carriers: 0.1% BSA or 5% HSA or 10% FBS.
SDS-PAGE analysis of recombinant human IL-1 Beta