Interleukin-10 is a multifunctional cytokine produced by a variety of cell types including T helper cells activated B cells and activated macrophages. Interleukin-10 regulates immune-mediated inflammation and modulates the function of cells such as lymphocytes, monocytes, natural killer cells and dendritic cells.

Expression System
Escherichia coli

Sequence
MSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA with polyhistidine tag at the C-terminus.

Species
Human

Tag
polyhistidine tag at the C-terminus

Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.

Activity
Measure by its ability to induce MC/9-2 cells proliferation.
The ED₅₀ for this effect is <1 ng/mL.
The specific activity of recombinant human IL-10 is approximately >1×10⁶ IU/ mg.

Purity
>98% as determined by SDS-PAGE. Ni-NTA chromatography

Formulation
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Reconstitution
Unopened ampoules can be stored at -20°C or -80°C.
Need not spin the ampoule. Open the cap carefully, then dissolve the lyophilized protein with sterile water for a 100 μg/mL concentration or according to the product’s Certificate of Analysis (CoA). Rinse the inner side of the ampoule gently and stand for at least 20 minutes at room temperature. (Important Note: Do Not Vortex!) Use the reconstituted solution immediately or store it by distributing it to aliquots and store at -20 °C. (Avoid repeated freeze-thaw cycles) We recommend diluting the solution to working concentration by using buffers or medium containing a carrier*. (Do not dilute the working solution with water, which may cause protein degradation.)
*Carriers: 0.1% BSA or 5% HSA or 10% FBS.

SDS-PAGE analysis of recombinant human IL-10