GeneDireX, Inc.

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Interleukin-2 (IL-2), Human

GMP Grade Available!


Size: 100 μg


Storage
Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.


Note
Please use within one month after protein reconstitution.

Cat.No. SR104-0100 Categories ,

IL-2 is a secreted cytokine that regulates the proliferation of T and B lymphocytes. The IL-2 receptor is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode to control the gene expression precisely. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.


Expression System
Escherichia coli


Sequence
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT with polyhistidine tag at the C-terminus


Species
Human


Tag
polyhistidine tag at the C-terminus


Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.


Activity
Measure by its ability to induce proliferation in CTLL-2 cells.
The ED50 for this effect is <0.2 ng/mL.
The specific activity of recombinant human IL-2 is approximately >2.5 x 10⁷ IU/mg.
Measure by its ability to induce proliferation in NK cells.
The ED50 for this effect is <46 ng/mL.


Purity
>98% as determined by SDS-PAGE. Ni-NTA chromatography


Formulation
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.


Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.


 

SDS-PAGE analysis of recombinant human IL-2

Protocol

SR104-0100
SR104-0100G

Document Searching (Protocol, CoA, SDS)

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