IL-3 is a potent growth promoting cytokine, which could support the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
MAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF with polyhistidine tag at the C-terminus
polyhistidine tag at the C-terminus
<0.1 EU per 1 μg of the protein by the LAL method.
Measure by its ability to induce TF-1 cells proliferation.
The ED₅₀ for this effect is <0.15 ng/mL.
The specific activity of recombinant human IL-3 is approximately >1.2 x 10⁶ IU/mg.
>98% as determined by SDS-PAGE. Ni-NTA chromatography
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.
SDS-PAGE analysis of recombinant human IL-3