GeneDireX, Inc.

KeraGo SFM Medium for Keratinocytes and Epithelial Cells

Size: 500 mL

Basal Medium Storage condition: 2-8°C, and should not be frozen, protect from light.
Growth Supplement Storage condition: -20°C or below, protect from light.

➣ To promote epithelial cells and keratinocytes proliferation in vitro.

Kit Contents

Contents Size
KeraGo SFM Medium 500 ml
KeraGo Growth Supplement 0.5 ml

Cat.No. CC818-0500 Categories ,

KeraGo SFM Medium is a serum-free, BPE (Bovine Pituitary Extract)-free, HPL (Human Platelet Lysate) -free, cholera toxin-free, chemically-defined culture medium for the growth and expansion of epithelial cells and keratinocytes without the use of feeder layers. KeraGo SFM Medium supports the primary culture of keratinocytes freshly isolated from tissues and the subsequent long-term culture. At the time of use, 500 mL of the basal medium of KeraGo is added with 0.5 mL of KeraGo Growth Supplement which contains recombinant human epidermal growth factor (rhEGF) and recombinant human insulin-like growth factor-1 (rhIGF-1). The calcium concentration in KeraGo SFM Medium is 60 μM. The complete medium of KeraGo contains essential and non-essential amino acids, lipids, vitamins, organic compounds, and inorganic salts. The medium is HEPES and sodium bicarbonate buffered. KeraGo SFM Medium does not contain antibiotics or antimycotics.

Storage and Stability
➣ KeraGo Basal Medium should be stored at 4°C protected from light and should not be frozen. The product is stable until the expiration date shown on the label when stored accordingly.
➣ KeraGo Growth Supplement should be stored at –20°C or below and protected from light. The product is stable until the expiration date shown on the label when stored accordingly.
➣ The complete medium (i.e. supplemented medium) of KeraGo should also be stored at 4°C in the dark without being frozen.
➣ Be sure that the freezer for storage does not undergo freeze-thaw cycles.

➣ Primary cell isolation and routine culture of epithelial cells and keratinocytes.

Quality Control
The quality of the KeraGo SFM Medium is tested on a lot-to-lot basis to ensure consistent product quality.