A 2X concentrated mix of Taq polymerase, dNTPs, MgCl2, fluorescent dye (detection), and proprietary buffer components, the SimplyGreen qPCR Master Mix, No Rox provides a convenient, reliable and robust set-up for performing quantitative real-time analysis of DNA samples. Designed specifically for the aforementioned niche of application, the components of Simp lyGreen qPCR Master Mix, No Rox promise topnotch performance with respect to sensitivity, signal-to-noise ratio and elimination of primer dimers. Furthermore, the proprietary chemical modification of t he DNA polymerase included in this mastermix allows for hot-start PCR, conferring a significant reduction in non-specific PCR amplification that is otherwise of a common occurrence with regular Taq polymerases.
Based on the fact that the qPCR instruments can vary from user to user, we suggest that the SimplyGreen qPCR Master Mix, No Rox is compatible with qPCR instrument: BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™; Roche LightCycler® (2.0, 1.5, 480, 1536,Nano); MJ Research Opticon™, Opticon™2, Chromo® 4; Corbett Rotor-gene® (3000,6200, 62H0, 6500, 65H0, 6600).
Applications
- Gene Expression (mRNA) Analysis
- Genetic Variation Analysis
- microRNA & Noncoding RNA Analysis