The MaxSYBR Green/ROX qPCR Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR. This ready-to-use qPCR Master Mix contains Hot Start Taq DNA polymerase and dNTPs in an optimized PCR buffer. It contains SYBR® Green I dye and is supplemented with ROX passive reference dye. The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil- DNA glycosylase (UDG). The use of MaxSYBR Green/ROX qPCR Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with real-time thermal cyclers, that are compatible with ROX reference dye.
Applications
- Gene expression (mRNA) analysis.
- Microarray validation.
Quality Control
The quality of the MaxSYBR Green/ROX qPCR Mix (2X), is tested on a lot-to-lot basis to ensure consistent product quality.
Required Materials
- Real-time PCR tubes
- Real-time PCR instrument
- Water, nuclease-free