Oligo(dT)₂₀ primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA and can be used as a primer for the first strand cDNA synthesis with the reverse transcriptase.
The primer is supplied in the DEPC water at a concentration of 50 μM.
cDNA synthesis with a reverse transcriptase.
The quality of the oligo (dT)₂₀ primer is tested on a lot-to-lot basis to ensure consistent product quality.
Equipments for reverse transcription
Oligo(dT)₂₀ primer Protocol
We recommend using 1 μl of oligo(dT)₂₀ primer per 20 μl reverse transcription reaction.