OnePCR™ Plus is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ Plus is a pre-mixed solution containing Taq DNA polymerase, PCR reaction buffers, dNTPs, gel loading dyes, and fluorescence dye. OnePCR™ Plus contains the Taq DNA polymerase, which is purified from the E. coli. and exhibits the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR™ Plus also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. OnePCR™ Plus mixture is supplied at the 2X concentrationto allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.
Store at room temperature up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping temperature: 4°C