GeneDireX, Inc.

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OnePCR™

Size: 100 Reactions (2 X 1.25 ml)

Features

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No destaining requirement.
  • Compatibility – Use the blue light or UV to detect the signal.

NT$1,500

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OnePCR™ is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.OnePCR™ which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR™, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR™ mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.Reagents are provided with the sufficient amplification reactions of 50 μl each.


Fig.1

Fig.1

Fig.1 OnePCR products are viewed using the Blue Light (left) or UV (right) to detect the signal.
Exposure to the Blue Light (left gel), and UV light (right gel)
Lane M: 1 kb Marker
Lanes 1~3: 1 kb, lanes 4~6: 2 kb, lanes 7~9: 4 kb
Template: p35s-GFP

 


Fig. 2

Fig. 2 PCR products of the genomic DNA (lane 1) or cDNA (lane 2) of Rose petal.
Exposure to the Blue Light.
Primer: Actin Forward Primer / Actin Reverse Primer
Lane M: 1 kb Marker

 

 

 

 

 


Fig. 3

 

Fig. 3 PCR products of the storage condition test for 3 months.
Exposure to the Blue Light.
M: 1 kb Marker
Lane 1: Room temperature for 3 months
Lane 2: 4°C for 3 months
Lane 3: -20°C for 3 months
Lane 4: 0 day

 

 


Fig. 4

Fig. 4 Sequence analysis . PCR products of both the general PCR(up) and OnePCR(down) from the p35s-GFP plasmid sequencing results exhibited as the same.

 

 

 

 

 

 

 

 

 

 

 


Fig. 5

Fig. 5 Results of the colony PCR separation on the 1.2% agarose gel.
Exposure to the UV light
Lane M: 1 kb Marker
Target: Lanes 1~3 : 1kb
Lanes 4~6: 4kb
Lanes 7~ 9 : 2kb
Template: p35S-GFP Plasmid

 

 

 

 


Fig. 6

Fig. 6 Cloning test of the OnePCR and general PCR products.After ligation , perform the PCR check on the insert.
Exposure to the Blue Light.
Insert: General PCR and OnePCR reaction products .
Primer : M13 Forward Primer / M13 Reverse Primer
Lane M: 1 kb Marker
Lane 1: General PCR
Lane 2: OnePCR

 

 

 

 

 


 

Storage
Store at RT up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping Temperature: 4°C
Note: OnePCR™ is light sensitive and should be stored and protected from light.


Application
◆  No post-staining processing of DNA required.
◆  No need to prepare PCR Reagents.
◆  Direct loading onto your agarose gel for analysis.
◆  Sensitivity – High degree of sensitivity as the ethium bromide.
◆  Speed – No de-staining requirement.
◆  Compatibility – Use the blue light or UV to detect the signal.


 

Mammalian-Chromosomal-Aberration Test
OnePCR-Acute-Toxicity Report
OnePCR-Mutation-Test Report

Protocol

MB203-0100

SM203-0100

MSDS

MB203-0100_MSDS

SM203-0100_MSDS

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