PCR SuperMix provides qualified reagents for the amplification of nucleic acid templates by the polymerase chain reaction (PCR). PCR SuperMix contains Mg++, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient to allow amplification during PCR. PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 μl each are provided. PCR SuperMix may be stored at either -20°C or 4°C. Storage at 4°C avoids the necessity of thawing the mix before assembling the PCR. No detectable reduction of PCR performance or enzyme activity is observed after storage of PCR SuperMix for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity.
PCR SuperMix is evaluated in a DNA polymerization activity assay that measures the percent of Taq DNA polymerase inhibition versus an uninhibited control. A functional assay is also performed. Components of PCR SuperMix are tested for the absence of DNase, RNase and exonuclease activities. Recombinant Taq DNA polymerase is tested for the absence of exonuclease, and double- and single-stranded endonuclease activities. The enzyme is >90% homogeneous as determined by SDSpolyacrylamide gel electrophoresis.