The PCR Clean-Up & Gel Extraction Kit provides a fast, easy, and cost-effective f system to isolate the DNA fragments from PCR reactions, agarose gels, or enzymatic re actions. The DNA fragments (100bp-10Kb) in the special buffers are bound by the glass fibe r matrix of the spin column while contaminants pass through the column. Impurities are efficientl y washed away, and the pure DNA is eluted with the Tris buffer or water without phenol extraction or alcohol precipitation. The DNA purified with the kits is suitable for any subsequent application, such as ligation and transformation, sequencing, restriction enzyme digestion, labeling, PCR, in vitro transcription, or microinjection. The entire procedure can be completed within 15-20 minutes.
- Quantity of DNA needed.
- Purity of DNA required.
The quality of the PCR Clean-Up & Gel Extraction Kit is tested on a lot-to-lot basis to ensure consistent product quality.