GeneDireX, Inc.

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Plasmid maxiPREP Kit

Sample: Up to 200 ml bacterial cells
Yield: Up to 850 μg of plasmid
Endotoxin value: <0.003 EU/μg
Operation time: Within 45 minutes
Elution volume: 2 ml

Kit Contents

ContentsSize
Buffer M185 ml X 1 bottle
Buffer M285 ml X 1 bottle
Buffer M3125 ml X 1 bottle
Buffer W1105 ml X 1 bottle
Buffer W2 (Add ethanol)25 ml x 1 bottles (100 ml X 1 bottles)
Buffer BE30 ml X 1 bottle
RNase A (50mg/mL)200 μl
MX Column10 pieces X 1 bag

NT$99,999

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The Plasmid maxiPREP Kit provides a fast, simple, and cost-effective method for the plasmid DNA isolation from the cultured bacterial cells. The Plasmid maxiPREP Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid maxiPREP Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.

Features
➤ Ready-to-use DNA for high performance in any downstream application.
➤ Consistent DNA yields from a small amount of the starting material.
➤ Time flexibility.

Applications
➤ Quantity of DNA needed.
➤ Molecular weight and size of DNA.
➤ Purity of DNA required.

Quality Control
The quality of the Plasmid maxiPREP Kit is tested on a lot-to-lot basis to ensure consistent product quality.
Required Materials
➤ Ethanol (96-100%)
➤ Microcentrifuge tubes
➤ Swing bucket rotor centrifuge

Buffer Preparation
➤ Add the provided RNase A solution to the Buffer M1, mix, and store at 2-8°C.

Protocol

NA305-0010

SN305-0010