Description
The QStrip One-Step RT-qPCR Mix has been freeze-dried to a bead format. Beads are stable at room temperature and designed to perform a fast and easy methods for one-step reverse transcription real-time PCR (RT-qPCR). The QStrip One-Step RT-qPCR Mix is provided in 0.1 ml tubes containing one lyophilized bead for one reaction. The lyophilized bead contains reverse transcriptase, DNA polymerases, dNTPs and buffer components at optimal concentrations. The additional reagents required are water, primers, and template RNA. The supermix is applicable for TaqMan probes system. The reverse transcriptase is modified from that be able to tolerate high temperature to 61°C, which is helpful for reverse transcription of RNA templates to unravel the complex RNA secondary structures. The specific DNA polymerase allows for high specificity and reproducible amplification.
Quality Control
Each lot of QStrip One-Step RT-qPCR Mix is functional test to ensure lot-to-lot reproducibility