The R-PE Labeling Kit provides a convenient way to conjugate an tibodies to R-Phycoerythrin (R-PE). R-PE belongs to the Phycobiliproteins family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. R-PE is made of α, β and γ subunits and is present as (αβ)6γ. The protein has broad absorption bands with peaks at 496 nm, 546 nm, and 565 nm, and can be excited with versatile excitation sources. The broad excitation spectrum provides the advantage for multi-color immunofluorescence staining and cell sorting. The R-PE Labeling Kit is a SH-reactive labeling kit. Modified R-Phycoerythrin can easily react with SH groups of a t arget antibody without the need for an additional activation, thus simplifying the conjugation protocol. Modified R-PE and sulfhydryl group on the antibody form a covalent bond during conjugation.
Figure 1. Physical and Spectral Properties of Activated R-PE:
Molecular weight: 240 kDa
Absorption peaks: 499, 541, 565 nm
Maximum emission: 578 nm
— Absorption
— Emission
Storage: 4°C (Do NOT freeze!)
Simple Procedures
Performance Tests
Recommended Kit Sizes for Antibody
Cat. No. |
Labeling Amount of Antibody | Recommended Amount of Antibody | Fixed Volume of Antibody | |
Minimum (0.5 μg/mL) |
Maximum (1 μg/mL) |
|||
RPE01-0010 |
10 μg |
5 μg | 10 μg (optimal) |
10 μL |
RPE01-0100 |
100 μg |
50 μg | 100 μg (optimal) |
100 μL |
Related Products
Cat. No. Product Name Size
RPE01-0010 R-PE Labeling Kit 10 ug
RPE01-0100 R-PE Labeling Kit 100 ug
RPE02-1000 R-PE (R-Phycoerythrin) 1 mg
RPE02-5000 R-PE (R-Phycoerythrin) 5 mg
RPE03-1000 SMCC-Activated R-PE 1 mg
RPE03-5000 SMCC-Activated R-PE 5 mg