GeneDireX, Inc.

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R-PE Labeling Kit

Size: 100 ug


  • Store all kit components at 4°C upon arrival.
  • Keep Activated R-PE in the dark, do not freeze.
  • Components B and D may be frozen (-20°C) for best performance.
  • The performance of the product is guaranteed for a minimum of 12 months when stored as directed.

Features

  • This kit is optimized for conjugating R-phycoerythrin (R-PE) to antibody.
  • It provides sample materials to perform one conjugation reaction.

Cat.No. RPE01-0100 Category

The R-PE Labeling Kit provides a convenient way to conjugate an tibodies to R-Phycoerythrin (R-PE). R-PE belongs to the Phycobiliproteins family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. R-PE is made of α, β and γ subunits and is present as (αβ)6γ. The protein has broad absorption bands with peaks at 496 nm, 546 nm, and 565 nm, and can be excited with versatile excitation sources. The broad excitation spectrum provides the advantage for multi-color immunofluorescence staining and cell sorting. The R-PE Labeling Kit is a SH-reactive labeling kit. Modified R-Phycoerythrin can easily react with SH groups of a t arget antibody without the need for an additional activation, thus simplifying the conjugation protocol. Modified R-PE and sulfhydryl group on the antibody form a covalent bond during conjugation.


Figure 1. Physical and Spectral Properties of Activated R-PE:
Molecular weight: 240 kDa
Absorption peaks: 499, 541, 565 nm
Maximum emission: 578 nm
Absorption
Emission
Storage: 4°C (Do NOT freeze!)


Simple Procedures


Performance Tests


Recommended Kit Sizes for Antibody

Cat. No.

Labeling Amount of Antibody Recommended Amount of Antibody Fixed Volume of Antibody
Minimum (0.5 μg/mL)

Maximum (1 μg/mL)

RPE01-0010

 10  μg

 5  μg  10  μg (optimal)

 10  μL

RPE01-0100

100 μg

50 μg 100 μg (optimal)

100 μL

 

Related Products

Cat. No.Product NameSize
RPE01-0010R-PE Labeling Kit10 ug
RPE01-0100R-PE Labeling Kit100 ug
RPE02-0010R-PE (R-Phycoerythrin)10 ug
RPE02-0100R-PE (R-Phycoerythrin)100 ug
RPE03-0010SMCC-Activated R-PE10 ug
RPE03-0010SMCC-Activated R-PE100 ug

Protocol

RPE01-0010

RPE01-0100

Document Searching (Protocol, CoA, SDS)

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