Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction.
We recommend using 1 μl of Random Hexamers primer per 20 μl reaction volume.
This product is qualified in a cDNA reaction.
- cDNA synthesis using a Reverse Transcriptase with RNA templates.
- DNA synthesis using the Klenow fragment with DNA templates.
- DNA probe synthesis for use in Southern, Northern, and in situ hybridization applications.
For research use only.