RiboIN™ RNase Inhibitor is a protein which specifically inhibits ribonucleases. It is used in applications such as in vitro translation, cDNA synthesis, RNA in vitro synthesis, RNA purifications, etc. RNase inhibitor is easier to use and eliminate than the vanadyl ribonucleosides. It has a high binding affinity for pancreatic-type ribonucleases such as RNase A.
RiboIN™ RNase Inhibitor inhibits a broad range of RNases, including RNase A, RNase B, RNase C, but it is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H.
RiboIN™ RNase Inhibitor is purified by affinity chromatography which expressing a cloned porcine liver gene from a recombinant strain of E. coli strain containing an overproducing clone of human placenta ribonuclease inhibitor
20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM EDTA, 8 mM DTT, and 50% (v/v) glycerol.
Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.
RiboINTM RNase Inhibitor requires 1 mM DTT to maintain the activity.
One Unit Definition
One unit is the amount of protein required to inhibit the activity of 5 ng of RNase A by 50%.
RiboIN™ RNase Inhibitor has been experimented in 12.5% SDS-PAGE electrophoresis.
It’s greater than 90% in purity. The specific activity is >80,000 units/mg.
cDNA Synthesis: 40 units/20 μl of reaction mixture, RiboIN™ RNase Inhibitor protects mRNA and improves total cDNA yields including percent total full length of cDNA.
RT-PCR: 40 units/20 μl of reaction mixture.
In Vitro Transcription: 20-40 units/10 μl of reaction mixture, RiboIN™ RNase Inhibitor has been shown to be useful for the isolation of intact RNA transcripts using T3, T7 and SP6 RNA Polymerases.
in vitro RNA transcription
in vitro protein synthesis
cDNA preparation by reverse transcription
Separation and identification of specific ribonuclease activities